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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 1860-1866
DOI: https://doi.org/10.20546/ijcmas.2021.1003.234


Effect of L-proline Supplementation in Vitrification Media on Post Thaw Survival and Embryonic Development of Sheep Oocytes
D. Reena1, G. Lakshmi2, S. Kurunchi Malar2 and S. Manokaran3*
1Department of Clinics, Madras Veterinary College, TANUVAS, Chennai, India
2Department of Biotechnology, Sree Sastha Institute of Engineering and Technology, Chembarambakkam, Chennai, India
3Kangayam Cattle Research Station, TANUVAS, Uppupallam, Baguthampalayam, Sathyamangalam, India
*Corresponding author
Abstract:

The study was conducted to explore the cryoprotective effect of L-proline on sheep oocyte vitrification. In vitro matured sheep oocytes (n=836) were vitrified by open pulled straw method in vitrification solution consisting of 15 per cent (v/v) DMSO+ 15 per cent (v/v) EG + sucrose (0.5M) + 20 per cent FBS in TCMh (Group I) and 7.5 per cent (v/v) DMSO + 10 per cent (v/v) EG + sucrose (0.5M) + 20 per cent FBS in TCMh supplemented with 2M L-proline (Group II). The oocytes were warmed and observed for viability by tryphan blue staining. The mean ± SE live oocyte percentage was significantly higher in group II than in group I (59.80±1.02 vs. 44.99±1.34). The vitrified thawed oocytes were activated parthenogenitically using ionomycin and 6-DMAP and developmental stages of embryos were analysed. In parthenogenetically activated embryos, there was no significant difference was observed in cleavage rate (47.56±2.09 vs/ 42.45±3.44) and morula (14.90±1.68 vs. 12.95±2.25) percentage between L-proline and control group. From the study it is concluded that L-proline could be used as a cryoprotectant with high efficiency in sheep oocyte vitrification.


Keywords: L-proline, Sheep oocyte, Vitrification, Parthenogenesis, Tryphan blue
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How to cite this article:

Reena, D., G. Lakshmi, S. Kurunchi Malar and Manokaran, S. 2021. Effect of L-proline Supplementation in Vitrification Media on Post Thaw Survival and Embryonic Development of Sheep Oocytes.Int.J.Curr.Microbiol.App.Sci. 10(3): 1860-1866. doi: https://doi.org/10.20546/ijcmas.2021.1003.234