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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:3, March, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 1649-1665
DOI: https://doi.org/10.20546/ijcmas.2021.1003.206


Mutagenesis of Trichoderma asperellum for Fungicide Tolerance
G. Gathe*, S. T. Ingle, S. S. Mane, S. B. Brahmankar and M. N. Ingole
Department of Plant Pathology, Dr. PDKV, Akola-444104, M.S., India
*Corresponding author
Abstract:

Mutagenesis of Trichoderma asperellum through chemical and physical means and tolerance of T. asperellum mutants to commonly used fungicides was carried out at Department of Plant Pathology, Dr. PDKV, Akola. The treatments with chlorothalonil 50 per cent WP @ 0.25 per cent, carbendazim 50 per cent WP @ 0.1 per cent, propiconazole 25 per cent EC @ 0.1 per cent, thiophanate methyl 70 per cent WP @ 0.25 per cent and carboxin 37.5 per cent + thiram 37.5 per cent @ 0.25 per cent, recorded complete growth inhibition of mutants and mother culture of T. asperellum. Mycelial growth of mutants and mother culture in the range of 20 to 85 mm was recorded in azoxystrobin 23 per cent SC @ 0.1 per cent. It proved that fungicide azoxystrobin 23 per cent SC @ 0.1 per cent concentration was more compatible with T. asperellum mutants and mother culture. Among the mutants, TaU4(T12) was recorded 84.4 mm mycelial growth with azoxystrobin 23 per cent SC @ 0.1 per cent at 7 DAI, where as TaU1(T11), TaG2(T14), TaU2(T10) and TaG1(T13) recorded 80.5, 79.9, 75.8 and 75.00 mm growth respectively. The mother culture TaMc (T17) recorded 29.1 mm growth.


Keywords: Mutagenesis, Fungicides, Chlorothalonil, Carbendazim, Propiconazole, Thiophanate methyl, Carboxin, Thiram and azoxystrobin

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How to cite this article:

Gathe, A. G., S. T. Ingle, S. S. Mane, S. B. Brahmankar and Ingole, M. N. 2021. Mutagenesis of Trichoderma asperellum for Fungicide Tolerance.Int.J.Curr.Microbiol.App.Sci. 10(3): 1649-1665. doi: https://doi.org/10.20546/ijcmas.2021.1003.206
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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