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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 1347-1357
DOI: https://doi.org/10.20546/ijcmas.2021.1003.165


In vitro Organogenesis from Nodal Derived Callus in Carnation (Dianthus caryophyllus L.) cv. ‘Master’ and Assessment of Genetic Fidelity of Acclimatized Plantlets
Kalpna Thakur* and Kamlesh Kanwar
Department of Biotechnology, Dr Y.S. Parmar University of Horticulture and Forestry, Solan, H.P., India
*Corresponding author
Abstract:

An efficient protocol for organogenesis and plant regeneration has been developed for Dianthus caryophyllus L. cv. ‘Master’ using nodal explant. Optimal callus formation (79.17%) from nodal explants was obtained on MS medium supplemented with 1.5 mg/l 2,4-D and 1.0 mg/l NAA. The highest shoot induction response (65.28%) was recorded on MS medium supplemented with 1.5 mg/l TDZ, 0.25 mg/l Kinetin and 0.25 mg/l NAA leading to maximum average no. of shoots per callus clump (5.14) and shoot length (1.43 cm). The highest percentage of root regeneration (84.44%) was observed on MS medium supplemented with 1.0 mg/l IBA and 100 mg/l activated charcoal. The in vitro raised plantlets were successfully acclimatized under ex vitro conditions and grew well in the glasshouse. All the regenerated plantlets appeared normal with respect to morphological characteristics with 100% survival rate. Somaclonal variation in tissue culture is a common phenomenon which makes it mandatory to check for genetic stability of plants. The genetic variation in the in vitro raised plants was confirmed using Inter Simple Sequence Repeats(ISSR) markers. Comparison of the bands with the mother plant revealed that the genetic variation frequency reached 6.54 % using fifteen ISSR markers.


Keywords: Carnation, In vitro, Nodal explants, Organogenesis, ISSR markers
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How to cite this article:

Kalpna Thakur and Kamlesh Kanwar. 2021. In vitro Organogenesis from Nodal Derived Callus in Carnation (Dianthus caryophyllus L.) cv. ‘Master’ and Assessment of Genetic Fidelity of Acclimatized Plantlets.Int.J.Curr.Microbiol.App.Sci. 10(3): 1347-1357. doi: https://doi.org/10.20546/ijcmas.2021.1003.165