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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 1276-1281
DOI: https://doi.org/10.20546/ijcmas.2021.1003.154


Comparison of Teicoplanin E-strip and Agar Dilution Methods against Clinical Isolates of Staphylococcus Species in a Rural Tertiary Care Centre
Megha*, D. Venkatesha and D. C. Shwetha
Department of Microbiology, Adichunchanagiri Institute of Medical Sciences,B.G.Nagara, Mandya, India
*Corresponding author
Abstract:

Staphylococcus are among the most frequently isolated organisms in clinical microbiology laboratories. Methicillin- resistance among Staphylococcus species has led to utilization of glycopeptides (Vancomycin and Teicoplanin) as the drug of choice for the treatment. Resistance to Teicoplanin is emerging among Staphylococcus species due to improper usage of this drug. Detection of Teicoplanin resistance is mainly done by disc diffusion method in the laboratories. Objectives are to detect and compare the resistant pattern of Teicoplanin by E-strip and agar dilution method of Staphylococcus species. Total of 100 samples of Staphylococcus species was included in the study. Teicoplanin resistant pattern were detected by E-strip method (Hi media) and by agar dilution method (Dilution range 0.5mg/ml to 128mg/ml) Results: Out of 100 Staphylococcus species, all the isolates were sensitive by E-strip method for Teicoplanin. By agar dilution method 95 were sensitive (≤8µg/ml), 2 were intermediate (≥16µg/ml) and 3 were resistant (≥32µg/ml) for Teicoplanin. Teicoplanin resistance detection by conventional disc diffusion method is difficult because of limited diffusion of its large molecule in agar (4). Results obtained in this study by comparing E-strip and Agar dilution is 95% concordant. Agar dilution method which is considered gold standard showed better performance compared to E-strip method, this could be due to different performance of commercially available gradient strips against staphylococci


Keywords: Teicoplanin,E-Strip, Agar dilution, Staphylococcus species
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How to cite this article:

Megha, S., D. Venkatesha and Shwetha, D. C. 2021. Comparison of Teicoplanin E-strip and Agar Dilution Methods against Clinical Isolates of Staphylococcus Species in a Rural Tertiary Care Centre.Int.J.Curr.Microbiol.App.Sci. 10(3): 1276-1281. doi: https://doi.org/10.20546/ijcmas.2021.1003.154