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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 1206-1219
DOI: https://doi.org/10.20546/ijcmas.2021.1003.148


Comparison of Transcriptome of Human Hepatocytes Experimentally Infected with Yellow Fever Virus Genotypes
Gustavo Moraes Holanda1,4*, Walter Félix Franco Neto1, Jardel Fábio Lopes Ferreira2, Francisco Canindé Ferreira de Luna2, Murilo Tavares Amorim1,João Pacifico Bezerra Neto3, Samir Mansour Moraes Casseb1,Juarez Antônio Simões Quaresma4 and Ana Cecília Ribeiro Cruz1
1Arbovirology and Hemorrhagic Fevers Section, Evandro Chagas Institute, Highway BR-316, km 7 s.n, Levilândia, PA, Brazil, 67030-000
2Institute of Biological Sciences, Federal University of Pará, S. Augusto Corrêa, 01-Guamá, Belém-PA, Brazil, 66075-110
3Biological Sciences Center, genetics department, Federal University of Pernambuco, Recife-PE, Brazil, 50730-120
4Post-graduate Program in Amazon Parasitic Biology, State of Pará University, Tv. Angustura, 2219-Pedreira, Belém-PA, Brazil, 66087-310
*Corresponding author
Abstract:

Analysis and comparison of the transcriptome of human hepatocytes infected with the Yellow Fever virus to verify the transcriptional profile of both genotypes, in response to viral infection. The sample used corresponds to strains of YFV code H622205, from the state of Goiás-GO (Brazil), a fatal human case isolated from the patient's blood and serum. The techniques of cell cultures, extraction of viral RNA, sequencing and analysis of transcriptome, as well as the pre-processing of both the RNA and transcriptome libraries, mapping and abundance of transcripts and statistical analyzes were all performed in the Arbovirology Section of the Institute Evandro Chagas.  According to the viral titration, there is a greater replication of Genotype II compared to Genotype I. In both infections, we noticed that the moment of the lowest viral load is 24 hpi and the highest viral load is 96 hpi. Regarding the VFA genotype II infecting HepG2, the same tests were carried out, but the significance of the statistical test for differential expression was less than that found in genotype II.A statistical test of formation of “HeatMap” was also performed by the RSEN software. The present study emphasize that this work has great value in clarifying differences in the infection of genotype I and II of YFV in human hepatocytes, in addition to being a milestone, for presenting the first transcriptome of both genotypes of YFV performed in hepatocytes.


Keywords: Immune response, Transcriptome, Viral infection, Yellow fever
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How to cite this article:

Gustavo Moraes Holanda, Walter Félix Franco Neto, Jardel Fábio Lopes Ferreira, Francisco Canindé Ferreira de Luna, Murilo Tavares Amorim, João Pacifico Bezerra Neto, Samir Mansour Moraes Casseb, Juarez Antônio Simões Quaresma and Ana Cecília Ribeiro Cruz. 2021. Comparison of Transcriptome of Human Hepatocytes Experimentally Infected with Yellow Fever Virus Genotypes.Int.J.Curr.Microbiol.App.Sci. 10(3): 1206-1219. doi: https://doi.org/10.20546/ijcmas.2021.1003.148