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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 740-747
DOI: https://doi.org/10.20546/ijcmas.2021.1003.094


Gamma Radiation Induced Mutagenesis in Trichoderma reesei to Enhance Chitinase Enzyme Activity
Kasani Sudharsanababu, M. V. Totawar, Payal V. Kose*,V. Zope and Sarika W. More
Department of Plant Pathology, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra, India
*Corresponding author
Abstract:

Trichoderma reesei is a widely distributed soil fungus that antagonizes numerous fungal phytopathogens. Random induced mutation by gamma radiation with five treatments TrME 1 (50 Kr), TrME 2 (100 Kr), TrME 3 (150 Kr), TrME 4 (200 Kr), TrME 5 (untreated) were studied for morphological study and chitinase activity. The result indicates that TrME 1 contain maximum i.e. 0.3201 chitinase enzyme units/mg of protein followed by TrME 4 recording 0.3108 chitinase enzyme units/mg of protein. This indicates that treatments TrME 1 and TrME 4 are the best mutants for use as effective mycoparasite in the biocontrol of plant pathogenic fungi.


Keywords: Trichoderma reesei, mutation, Chitinase enzyme, CDR Design, Potato dextrose agar
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How to cite this article:

Kasani Sudharsanababu, M. V. Totawar, Payal V. Kose, A. V. Zope and Sarika W. More. 2021. Gamma Radiation Induced Mutagenesis in Trichoderma reesei to Enhance Chitinase Enzyme Activity.Int.J.Curr.Microbiol.App.Sci. 10(3): 740-747. doi: https://doi.org/10.20546/ijcmas.2021.1003.094