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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
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Original Research Articles                      Volume : 10, Issue:3, March, 2021
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
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Int.J.Curr.Microbiol.App.Sci.2021.10(3): 740-747
DOI: https://doi.org/10.20546/ijcmas.2021.1003.094


Gamma Radiation Induced Mutagenesis in Trichoderma reesei to Enhance Chitinase Enzyme Activity
Kasani Sudharsanababu, M. V. Totawar, Payal V. Kose*,V. Zope and Sarika W. More
Department of Plant Pathology, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra, India
*Corresponding author
Abstract:

Trichoderma reesei is a widely distributed soil fungus that antagonizes numerous fungal phytopathogens. Random induced mutation by gamma radiation with five treatments TrME 1 (50 Kr), TrME 2 (100 Kr), TrME 3 (150 Kr), TrME 4 (200 Kr), TrME 5 (untreated) were studied for morphological study and chitinase activity. The result indicates that TrME 1 contain maximum i.e. 0.3201 chitinase enzyme units/mg of protein followed by TrME 4 recording 0.3108 chitinase enzyme units/mg of protein. This indicates that treatments TrME 1 and TrME 4 are the best mutants for use as effective mycoparasite in the biocontrol of plant pathogenic fungi.


Keywords: Trichoderma reesei, mutation, Chitinase enzyme, CDR Design, Potato dextrose agar

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How to cite this article:

Kasani Sudharsanababu, M. V. Totawar, Payal V. Kose, A. V. Zope and Sarika W. More. 2021. Gamma Radiation Induced Mutagenesis in Trichoderma reesei to Enhance Chitinase Enzyme Activity.Int.J.Curr.Microbiol.App.Sci. 10(3): 740-747. doi: https://doi.org/10.20546/ijcmas.2021.1003.094
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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