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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 725-732
DOI: https://doi.org/10.20546/ijcmas.2021.1003.092


Molecular Detection and Characterization of Mycoplasma Species from Respiratory Infections of Small Ruminants in Namakkal District of Tamil Nadu
Ponnusamy1*, K. Sukumar1, A. Raja2, S. Saravanan1 and P. Srinivasan3
1Dept. of Veterinary Microbiology, 2Education Cell, 3Dept. of Veterinary Pathology, Veterinary College and Research Institute, Namakkal, India
*Corresponding author
Abstract:

The present study aimed to identify and characterize the Mycoplasma species associated with respiratory infections of small ruminants by molecular method. A total of 61 samples including nasal swabs, lung swabs and heart blood swabs were collected from 11 different sheep and goat farms in and around Namakkal district of Tamil Nadu with the history of respiratory manifestations. The molecular PCR was employed to amplify the 16S rRNA gene of Mycoplasma species and M.mycoides cluster PCR and also species specific primer of M. capricolum subsp. capri pneumoniae (Mccp). Nasal and lung swabs of five farms were found to be positive by PCR for Mycoplasma genus specific 16S rRNA gene with a size of 714 bp. Mycoplasma genus specific positive samples were negative for M. mycoides cluster PCR and Mccp. The positive purified PCR amplicons from five representative samples were subjected for sequencing. Out of which, four samples from sheep were closely related with Mycoplasma arginini and one sample from goat was closely related with Mycoplasma conjunctivae. The study provides rapid detection, specificity and sensitivity compared to conventional isolation and identification methods which are time consuming, laborious and expensive.


Keywords: Mycoplasma species, M. arginini, M. conjunctivae, Small ruminants and PCR
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How to cite this article:

Ponnusamy, P., K. Sukumar, A. Raja, S. Saravanan and Srinivasan, P. 2021. Molecular Detection and Characterization of Mycoplasma Species from Respiratory Infections of Small Ruminants in Namakkal District of Tamil Nadu.Int.J.Curr.Microbiol.App.Sci. 10(3): 725-732. doi: https://doi.org/10.20546/ijcmas.2021.1003.092