National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study aimed to identify and characterize the Mycoplasma species associated with respiratory infections of small ruminants by molecular method. A total of 61 samples including nasal swabs, lung swabs and heart blood swabs were collected from 11 different sheep and goat farms in and around Namakkal district of Tamil Nadu with the history of respiratory manifestations. The molecular PCR was employed to amplify the 16S rRNA gene of Mycoplasma species and M.mycoides cluster PCR and also species specific primer of M. capricolum subsp. capri pneumoniae (Mccp). Nasal and lung swabs of five farms were found to be positive by PCR for Mycoplasma genus specific 16S rRNA gene with a size of 714 bp. Mycoplasma genus specific positive samples were negative for M. mycoides cluster PCR and Mccp. The positive purified PCR amplicons from five representative samples were subjected for sequencing. Out of which, four samples from sheep were closely related with Mycoplasma arginini and one sample from goat was closely related with Mycoplasma conjunctivae. The study provides rapid detection, specificity and sensitivity compared to conventional isolation and identification methods which are time consuming, laborious and expensive.
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