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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 324-331
DOI: https://doi.org/10.20546/ijcmas.2021.1003.043


Molecular Characterization of Germplasm Accessions of hirsutum using SSR Markers
Mayuri Tamboli, Sushil Mawle, M. Saravanan, S. S. Mahajan and V. N. Waghmare*
ICAR-Central Institute for Cotton Research, Nagpur, Maharashtra, India
*Corresponding author
Abstract:

The present investigation studied genetic diversity of ninety four cotton germplasm accessions, in order to improve genetic base of the present cultivars. Out of 48 primer pairs surveyed, 20 (42%) were showing polymorphism. The average number of alleles per locus was 2.45. The polymorphism information content (PIC) varied from 0.0735 to 0.6461 with an average value of 0.2978. The major allele frequency ranged from 0.3352 to 0.9602 with the mean value of 0.7304. The observed heterozygosity values for the marker loci varied from 0.000 to 0.702 with the mean value of 0.146. Three SSR loci namely MUSS0013, GH0499 and BNL2960 did not show heterozygosity/any heterozygotes while remaining loci showed the values ranging from 0.0118 to 0.7024. The expected heterozygosity (gene diversity) values varied from 0.0764 to 0.7046. NJ tree showed three major genotypic clusters within the collection of 94 accessions with bootstrap support. Cluster 1 (G1) was a major group consisting of 42 accessions with several subgroups. Cluster 2 (G2) included 33 accessions and cluster 3 (G3) included 19 accessions.


Keywords: Cotton, G. hirsutum, Molecular characterization, SSR markers
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How to cite this article:

Mayuri Tamboli, Sushil Mawle, M. Saravanan, S. S. Mahajan and Waghmare, V. N. 2021. Molecular Characterization of Germplasm Accessions of G. hirsutum using SSR Markers.Int.J.Curr.Microbiol.App.Sci. 10(3): 324-331. doi: https://doi.org/10.20546/ijcmas.2021.1003.043