National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The MEP pathway provides the initial precursors for the biosynthesis of terpenoids and carotenoid. Herein, we are reporting for the first time isolation, characterization and functional validation of the two isoforms of CfDXS gene from Coleus forskohlii. The full-length cDNAs of CfDXS1 and CfDXS2 were 2371 bp and 2541 bp, respectively having an open reading frame of 2148 bp and 2169 bp. The ORFs of CfDXS1 and CfDXS2 encoded protein of 715 and 722 amino acids, respectively, sharing 62.25% identity. Phylogenetic tree analysis classified the two isoforms in their respective plant clades. The CfDXS1 gene was constitutively expressed in all the tissues (root, stem and leaf) of plant whereas CfDXS2 gene expression was found to be higher in roots. The amino acid residues of the active site essential for the catalysis with the substrate, glyceraldehyde 3-phosphate, were found to be conserved in the CfDXS1 and CfDXS2 proteins. Identification of the function of CfDXS2 in the biosynthesis of carotenoid biosynthesis was confirmed using color complementation assay as it accelerated the biosynthesis of β-carotene, which was not as effective in CfDXS1. Therefore, our results suggested the differential role of CfDXS1 and CfDXS2 in the plant growth and development.
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