National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Toll-like receptors (TLRs) are type of pattern recognition receptors (PRRs) greatly expressed on all immune cells. Activation of TLRs with their cognate ligand initiates the induction of innate immune responses with subsequent priming of adaptive immunity. Understanding the interaction between two different TLRs in terms of induced immune responses would help in using the TLR ligands as vaccine adjuvants. In the present study, effects of Poly I:C (TLR3 ligand) and/or R-848 (TLR7 ligand) were studied in chicken peripheral blood mononuclear cells (PBMCs). The PBMCs were isolated from White Leghorn chickens of around six weeks of age (n=6) and stimulated with Poly I:C and R-848 alone or in combination. The cells were harvested at different intervals (3, 6, 12, 24 and 48 h) post-stimulation and analyzed for the induction of different immune response genes by real-time PCR. The combination of PolyI:C and R-848 synergistically induced the up-regulation of IL-1β, IFN-β, IFN-γ, IL-4, IL-10 and iNOS transcripts as compared to the individual response in the chicken PBMCs. The results indicate that the combination of Poly I:C and R-848 would synergistically interact at cellular signaling level resulting in augmentation of pro-inflammatory, antiviral, Th1 and Th2 type of responses in the chicken.
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