National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Mycoplasma gallisepticum (MG) is the most pathogenic avian mycoplasmas and is world-wide in distribution. It is economically significant pathogen causing Chronic Respiratory Disease in chicken and Infectious sinusitis in Turkeys. The current investigation deals with detection of MG infection in chicken and turkeys from the poultry flocks of Tirunelveli and Kanyakumari districts of Tamilnadu, India. Affected turkeys showed congestion of conjunctival mucous membrane, mild respiratory rales, nasal discharge, dullness and unilateral and/or bilateral swelling of infraorbital sinuses. Here we report 14.13% and 10.97% of morbidity and mortality, respectively among affected turkey. A total of 34 poultry samples including 11 samples from turkey collected during the period from March 2017 to February 2019 have been screened. The etiological agent was confirmed based on isolation, genus specific and species specific PCRs. The samples were cultured in PPLO agar and incubated at 37oC under 5% CO2 to reveal fried egg appearance under microscope. Further, the samples were subjected to genus specific PCR followed by MG species specific PCR which yielded specific amplicons of 715 bp and 185 bp, respectively in positive cases. The report supports the use of molecular PCR without the need for laborious culture identification for direct screening of field samples.
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