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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:4, April, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1796-1803
DOI: https://doi.org/10.20546/ijcmas.2017.604.215


Applicability of Yellow Pigmented Microbe obtained from Indian Rock Python Fecal Sample as Bio-Ink
J.R. Parvathi*, Shilpa Madhavan and R. Madhan Kumar
1School of Biotechnology and Bioinformatics, D. Y. Patil University, Level 5, Sector 15, Plot No. 50, CBD Belapur, Navi Mumbai, Maharashtra 400614, India
2Amity Institute of Biotechnology, Amity University, Mumbai, Maharashtra, India
3Bharathidasan University Constituent College, Perambalur, Tamil Nadu, India
*Corresponding author
Abstract:

A non-diffusable yellow pigmented isolate (Y_01) was isolated from a week-long enrichment of fecal sample of Indian rock python in cholesterol minimal medium. Basic biochemical characterization followed by 16S ribosomal DNA sequencing and Matrix Assisted Laser Desorption/Ionization method led to identification of the isolate as Micrococcus luteus. Nutrient broth (pH 7.4) supplemented with 2.5% NaCl was used as the growth medium with incubating conditions of 37ËšC for 24 hours as ideal scenario. The microbial pigment was found to be insoluble in most of the polar and non-polar solvents and resistant to both acid and alkali. The yellow pigment showed durable staining on both glossy and non-glossy papers with absence of spreading in the presence of alcohol and acetone, thus establishing applicability of the same as “bio-ink”.


Keywords: Bio-color, Micrococcus luteus, Bacterial pigments, Bio-ink.

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How to cite this article:

Parvathi, J.R., Shilpa Madhavan and Madhan Kumar, R. 2017. Applicability of Yellow Pigmented Microbe obtained from Indian Rock Python Fecal Sample as Bio-Ink.Int.J.Curr.Microbiol.App.Sci. 6(4): 1796-1803. doi: https://doi.org/10.20546/ijcmas.2017.604.215
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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