National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Brucellosis is an infectious disease, has a considerable impact on animal health as well as socioeconomic impacts. It causes significant reproductive losses in sexually mature animals. The present study reports the isolation, identification and molecular detection of Brucella abortus. A total of 192 buffalo clinical samples were processed by cultural isolation on BBL Brucella agar plate with selective antibiotic supplements and direct PCR. Out of 192 clinical samples, 7 samples growth yielded on BBL Brucella agar plate and identify Brucella organism by colony character, Gram’s staining, MZN staining, Catalase, KOH and Triple Sugar Iron Agar (TSI) test. Moreover, the confirmation of these isolate as Brucella abortus was carried out by genus specific PCR using B4/B5 (223bp), species specific PCR using +IS711 (498bp), and SYBR green based real time PCR. In direct PCR out of 192 clinical samples, 12 samples detected positive by genus specific PCR using B4/B5 (223bp). Genus specific PCR positive 12 samples conform Brucella abortus by species specific PCR using +IS711 (498bp), and SYBR green based real time PCR. In this study 7 clinical samples positive for B. abortus by culture isolation as well as direct PCR. However, 5 clinical samples positive by direct PCR but could not be growth yielded on BBL Brucella agar plate.
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