National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Xylooligosaccharides are emerging group of prebiotics that exert beneficial effects on human beings and other animals when supplemented with their diets as these are not digested in the upper gastrointestinal tract and help in proliferation of beneficial microbes. In the present study corn cobs were pretreated with dilute acid, dilute alkali and steam, for the production of xylooligosaccharides. The pretreatment methods were evaluated based on removal of lignin after the pretreatment and release of reducing sugars after saccharification with commercial xylanase. It was observed that, alkali pretreatment method was found to be best pretreatment method as it could remove maximum amount of lignin (5.7%). Also, alkali pretreated corncobs could able to release maximum amount (71.7±1.1mg per g of substrate) of reducing sugar upon saccharification than steam and acid pretreated corncobs. Hundred miligrams of xylobiose was obtained per g of alkali pretreated corncob (on dry weight basis) after 24hr using partially purified xylanase. Thus the present study proves the suitability of alkali pretreatment for the production of xylooligosaccahride from corn cob.
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