National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was carried out with an objective to find out most important autosomal recessive disorders in Gaolao cattle. Factor XI deficiency is one of the most important monogenic recessive disorder which causes prolonged bleeding, anemia, greater prevalence of repeat breeding or even lower resistance to pneumonia, mastitis and metritis resulting in reduced reproductive performance and increased susceptibility to disease. The only way to avoid economic losses is through an early detection of carriers. A total of 50 blood samples of Gaolao bull were collected from Gaolao bull mother farm, Hetikundi village of Wardha, Pohara farm of Amravati District and frozen semen lab, Nagpur of Maharashtra State. DNA extraction was done by using Phenol Chloroform Isoamyl Alcohol method. The isolated DNA was subjected for the quality and quantity with the help of gel electrophoresis and Nanodrop respectively. Then best quality DNA was selected for the Polymerase Chain Reaction. All the animals in present study showed only 244 bp band suggesting non occurrence of FXID gene in the studied population.
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