National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of this study was to determine the prevalence of extended spectrum beta lactamases (ESBLs) associated genes and integron elements in Escherichia coli from faeces of pigs in North eastern (NE) states of India. A total of 790 faecal samples were collected from pigs maintained under organized as well as individual house hold irrespective of age, sex and with or without history of diarrhea from all the eight states of NE region. A total of 2291 E. coli were isolated and identified. All the isolates were subjected to antimicrobial susceptibility test against 18 antimicrobial agents by disk diffusion method. The selected ESBLs genes (blaSHV, blaTEM, blaCMY and blaCTX-M) and integron (IntI1 and IntI2) genes were detected by specific PCR assay. A total of 366 (15.98%) and 80 (3.49%) isolates were positive for IntI1 and ESBLs, respectively. Twenty four (1.05%) isolates positive for IntI1 were carrying multiple ESBLs genes, and individually 0.17%, 1.92%, 0.17% and 0.17% isolates were positive for blaSHV, blaTEM, blaCMY and blaCTX-M genes, respectively. Class 2 integrons (IntI2) were not detected in any of the E. coli isolates under the study. It may be concluded that E. coli isolates with multiple ESBL genotypes have a greater opportunity to carry Class 1 integron and can be a potential to exhibit stronger multi-drug resistance activity.
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