National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was attempted with the purpose to introgress genes harboring big bollness to enrich gene pool of G. arboreum desi cotton popular varieties viz., AKA-7 (P1), Phule Dhanwantari (P2) from donor parent’s G. cernuum (D1) and PA-812 (D2). Four single cross hybrids namely SC-I (AKA - 7 x G. cernuum), SC-II (AKA-7 x PA-812), SC-III (PD x G.cernuum) and SC-IV (PD x PA-812) were attempted using above lines by manual emasculation and pollination. Accordingly, a total of 333 crosses were attempted, out of these, a total of 55 crosses made successful. Parental polymorphism and genetic diversity was assessed using 20 SSRs, where seven SSR found polymorphic. A dendrogram was constructed using Jaccard’s similarity coefficient and genotypes were grouped into three clusters based on the SSR profile. A lowest similarity coefficient value (0.80) was found between P1 and D1 indicated high genetic diversity occurred between them. The highest similarity coefficient value (0.90) in P2 and D2 was noticed, indicating low diversity exists between them. Among SSRs, only BNL-3580 showed the highest level of polymorphism 94%. From 55 successful, 18 single cross hybrids were confirmed as true hybrids by SSR genotyping proving SSRs as excellent genomic tools for parentage confirmation and hybridity determination.
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