National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The experiment was conducted at UBKV, Pundibari, Coochbehar during the academic year of 2016 – 2018 to find out the monokaryotic isolates of P. flabellatus, P. sajor-caju and P. djamor (oyster pink or OP) for its dikaryotization with the help of rapidity in the growth performance. Eighteen, six and five monokaryotic isolates of P. flabellatus (PFM), P. sajor-caju (PSCM) and P. djamor (OPM), respectively were grown on Malt Extract Agar media (MEA) for testing their rapidity in radial growth. After the selection of monokaryotic isolates growth performance was again tested for selection of dikaryotic culture on Malt Extract Agar (MEA) media. In MEA media, rapid radial growth of monokaryotic isolates viz., PF8M, PF11M, PF16M and PF17M for P. flabellatus, PSC3M and PSC5M for P. sajor-caju, OP1M and OP5M for P. djamor (oyster pink or OP) was finally selected for dikaryotization (hybridization). Highest growth rate on MEA was observed in interspecific hybrids of PF × PSC12 (18.50 mm/day), PF × PSC16 (18 mm/day), PF × PSC17 (17.50 mm/day), PF × PSC8 (17 mm/day) and PF × PSC2 (16.50 mm/day), respectively. Interspecific hybrids of OP × PSC5 (21.50 mm/day) followed by OP × PSC4 (21 mm/day), OP × PSC8 (20.50 mm/day) and OP × PSC12 (19.50 mm/day) and hybrids of OP × PF 5, OP × PF1, OP × PF2, OP × PF4 shows the growth rate above 18 mm/day. Dikaryotic mycelium showed highest growth rate as compared to monokaryotic isolates of P. flabellatus, P. sajor-caju and P. djamor (oyster pink).
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