National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The use of microbial pesticides in agriculture has someadvantages, but their production ina non-conventional experimental tests constituate a challenge. This study purpose is to evaluate the potential of cocoa mucilage juice. The mucilage juice of cocoais a substratum, that has been used as an alternative growing test and associated to bacteria such as Bacillus thuringiensis var. kurstaki HD-1 (Btk HD-1) for the production of a biopesticide. After determining the physico-chemical characteristics of the mucilage juice, different combinations has been made with 2% (M2%), 4% (M4%), 6% (M6%) and 7% (M7%) cane molasses were established, then inoculated with 2% of the inocula and incubated at 30 °C for 48 hours under agitation. The results showed that the substratum contained nitrogen (1.68g/L) and carbon (9.9 g/L), which has an important mineral such as potassium (87.3 mg/L), phosphorus (38 mg/L) and magnesium (25.42 mg/L). The growth of Btk HD-1 in cocoa mucilage juice with 2% molasses (M2%) was the highest (p < 0.0001) with average concentration of 1.6×109 CFU/mL for cells and 2.3×109 CFU/mL for spores. This combination therefore offers good prospects for the production of the Btk HD-1 biopesticide.
ACHI Stanislas Maurice Akpo, YAPO Atse Alain, DJENI N' dede Théodore, GADJI Alahou André Gabaze, COULIBALY Kalpy Julien, GNEPE Jean Robert, YAPO Ossey Bernard and TYAGI Dayal Rajeshwar. 2020. Valorization of the Mucilage Juice of Cocoa Beans for the Production of a Biopesticide based on Bacillus thuringiensis var.kurstaki HD-1.
Int.J.Curr.Microbiol.App.Sci. 9(11): 3600-3610. doi: https://doi.org/10.20546/ijcmas.2020.911.431
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