National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Brucellosis is the most common zoonosis in the world, Brucellosis is an infectious disease caused by bacteria of the genus Brucella. The disease has a considerable impact on human and animal health, as well as socioeconomic impacts, especially in which rural income relies largely on livestock breeding and dairy products. The disease can also be transmitted by direct or indirect contact with infected animals. Humans are commonly infected through ingestion of raw milk, milk product, meat or through direct contact with infected animals. In presence study was undertaken for the molecular detection of brucella organism from milk and milk products from different places of Gujarat. Out of 85 milk samples, 23 (27.05%) samples were found positive for Brucella antibodies by Milk Ring Test (MRT). A total of 168 samples (145 milk product and 23 MRT positive milk samples) processed for detection of Brucella organism using genus specific (B4/B5 primer) PCR. Out of 168 samples, 14 samples amplicon at 223bp by Brucella genus specific B4/B5primers indicates that all fourteen samples positive for Brucella organism.
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