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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:11, November, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(11): 2261-2271
DOI: https://doi.org/10.20546/ijcmas.2020.911.271


Isolation, Biochemical and Molecular Characterization of Endophytic Bacteria from Tomato (Lycopersicon esculentum Mill.)
B. Sai Sushma*, B. Vidya Sagar, S. Triveni and G. Uma Devi
Department of Plant Pathology, Professor Jayashankar Telangana State Agricultural University (PJTSAU), Rajendranagar, Hyderabad, India
*Corresponding author
Abstract:

In the present study, 24 isolates of endophytic bacteria were obtained from different plant tissues including root, stem and fresh leaves regions of tomato plants cv. Arka Vikas. The study characterizes morphological, biochemical and molecular characters of isolated bacterial endophytes. Among twenty-four isolates, nineteen isolates were found positive to HCN production, seventeen isolates to IAA production, and seven isolates to siderophore production. The isolates that were found to be highly effective were characterized on molecular basis by 16Sr RNA gene sequencing and based on the sequencing, the endophytic isolates were identified as Bacillus xiamenensis (EBT8), Bacillus aerius (EBT14), Bacillus stratosphericus (EBT18) and Bacillus safensis (EBT22).


Keywords: Endophytic bacteria, Bacillus sp., 16S rRNA sequencing

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How to cite this article:

Sai Sushma, B., B. Vidya Sagar, S. Triveni and Uma Devi, G. 2020. Isolation, Biochemical and Molecular Characterization of Endophytic Bacteria from Tomato (Lycopersicon esculentum Mill.).Int.J.Curr.Microbiol.App.Sci. 9(11): 2261-2271. doi: https://doi.org/10.20546/ijcmas.2020.911.271
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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