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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
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Original Research Articles                      Volume : 9, Issue:11, November, 2020
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2020.9(11): 887-890
DOI: https://doi.org/10.20546/ijcmas.2020.911.106


Diagnosis of Equine Herpes Virus 4 Infection using Polymerase Chain Reaction
J. A. Vala1, M. D. Patel2*, D. R. Patel3, U. V. Ramani4, I. H. Kalyani3, P. H. Makwana3 and D. N. Desai3
1Veterinary Clinical Complex,
3Department of Veterinary Microbiology,
4Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, NAU, Navsari, India
2College of Agriculture, NAU, Waghai, India
*Corresponding author
Abstract:

EHV-4 is respiratory pathogen of domestic horses associated with outbreaks of respiratory disease. The present study was conducted to diagnose an EHV-4 infection among domestic horses using polymerase chain reaction. Total 12 nasal swabs were collected from horses showing symptoms of respiratory disease, unthrifyness and fever. DNA was extracted from all samples and it was subjected to polymerase chain reaction for identification of EHV-4 DNA in samples. Four samples found positive for having EHV-4 infection revealed single compact band of 189 bp. PCR has been proved as an effective, less time consuming and sensitive as well as specific diagnostic test for diagnosis of EHV-4 infection.


Keywords: EHV-4, Diagnosis, PCR

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How to cite this article:

Vala, J. A., M. D. Patel, D. R. Patel, U. V. Ramani, I. H. Kalyani, P. H. Makwana and Desai, D. N. 2020. Diagnosis of Equine Herpes Virus 4 Infection using Polymerase Chain Reaction.Int.J.Curr.Microbiol.App.Sci. 9(11): 887-890. doi: https://doi.org/10.20546/ijcmas.2020.911.106
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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