Studies on Callus Induction and in Vitro Production of Secondary Metabolite in Andrographis echioides (L.) Nees

Hemalatha; V. Sivakumar; K. Rajamani

doi:https://doi.org/10.20546/ijcmas.2020.910.432

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Original Research Articles Volume : 9, Issue:10, October, 2020

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Int.J.Curr.Microbiol.App.Sci.2020.9(10): 3756-3763

DOI: https://doi.org/10.20546/ijcmas.2020.910.432

Studies on Callus Induction and in Vitro Production of Secondary Metabolite in Andrographis echioides (L.) Nees

Hemalatha¹, V. Sivakumar² and K. Rajamani³

¹Agricultural Research Station, Tamil Nadu Agricultural University,
Bhavanisagar - 638 451, Tamil Nadu, India
²Coconut Research Station, Tamil Nadu Agricultural University,
Aliyarnagar - 642 101, Tamil Nadu, India
³Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore - 641 003, India

^*Corresponding author

Abstract:

The present research was focused in standardizing the callus induction technique and studies the behaviour of callus in secondary metabolite production in Andrographis echioides (L.) Nees. In this study, the leaf bits of Andrographis echioides responded positively for callus induction. Earliest (21.00 days) and profuse callusing was observed on MS medium supplemented with 2, 4-D (2.0 mgl^-1) + NAA (2.0 mgl^-1) + BAP (2.0
mgl^-1). The same treatment combination responded highly for callus proliferation also. Highest andrographolide content of 1.31 per cent was observed in callus grown in media supplemented with 2, 4-D (2.0 mgl^-1) + NAA (2.0 mgl^-1) + BAP (2.0 mgl^-1) combination. The alkaloid content registered an increasing trend from 45 days to 60 days of culturing.

Keywords: MS medium, Explant, Callus, Proliferation, Andrographolide

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How to cite this article:

Hemalatha, P., V. Sivakumar and Rajamani, K. 2020 Studies on Callus Induction and in Vitro Production of Secondary Metabolite in Andrographis echioides (L.) NeesInt.J.Curr.Microbiol.App.Sci. 9(10): 3756-3763. doi: https://doi.org/10.20546/ijcmas.2020.910.432

Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Original Research Articles Volume : 9, Issue:10, October, 2020

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