National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In present study, a previously fabricated tripolymer composite scaffold of PCL/gelatin/chitosan was modified with collagen I and the modified scaffold were studied for biocompatibility of L929 mouse fibroblasts and human osteoblast cells. Phase contrast microscopy confirmed the viability of L929 mouse fibroblasts and human osteoblasts cultured with the modified composite scaffold which indicated that scaffold did not produce any toxic effect on both cells. Giemsa staining demonstrated the fibroblastic characteristic morphology of L929 and osteoblastic characteristic morphology of human osteoblast cells. FE-SEM study illustrated the adhesion and proliferation of L929 fibroblasts and osteoblasts, and retained their characteristic morphology on the modified PCL/gelatin/chitosan composite scaffold. Therefore, these results indicated that both cells were viable, proliferating, and showing their phenotypic morphology on collagen I modified PCL/gelatin/chitosan composite nanofibrouss caffold. Hence, the collagen I modified PCL/gelatin/chitosan composite nanofibrouss caffold might be an acceptable contender for skin and bone tissue engineering applications.
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