National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was planned to develop the best fecal extraction method for progesterone metabolite assay which is based on the concentration of fecal progesterone metabolite (FPM) (5α-pregnan-3α-ol-20-one), obtained in a dried fecal sample of buffaloes. Four clinically healthy female buffaloes of the same age group 4 to 7 years were used for the collection of fecal samples maintained under isomanagerial conditions with an intensive system at the LPM Section of the IVRI institute. The extraction of FPM (5α-pregnan-3α-ol-20-one) was done by various methods using organic solvents like methanol, diethyl ether, ethanol, and 90% methanol and found that extraction with 90% methanol the concentration of FPM (5α-pregnan-3α-ol-20-one) (231.98ng/g), is comparatively higher than other methods and best for a dried fecal sample of buffaloes. In conclusion, the above results suggest that FPM (5α-pregnan-3α-ol-20-one) value using 90% methanol as a solvent in the dried fecal sample assay having high value as compared to other solvents extraction methods like methanol, absolute ethanol, and diethyl ether, and we reported best for a dried fecal sample of buffaloes and preserved for further estimation.
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