National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A study was carried to investigate the epidemiology and molecular characterization of Shiga toxigenic Escherichia coli (STEC) from diarrhoeic lambs in Andhra Pradesh. A total of 212 faecal samples from diarrhoeic lambs of 1-7, 8-30, 31-60 and 61-90 days age groups were collected from Vizianagaram, West Godavari, Krishna, districts of Andhra Pradesh. The E. coli isolates were confirmed by cultural, biochemical and molecular methods. The virulence genes of STEC were detected using multiplex PCR. The results of the study revealed that the prevalence rate of E. coli in diarrhoeic lambs was 80.18% of which 87.05 % were identified as STEC. Among the virulence genes of STEC studied, eaeA&hlyA genes were more prevalent (35.13%), followed by stx1 (12.83%) and stx2 (10.13%) genes. The combinations of stx1&eaeA, stx2& eaeA genes carrying isolates detected were 5.4% and 8.1%, respectively. Only four (2.70%) STEC isolates carried all the four (stx1, stx2, eaeA and hlyA) genes. This study indicated that diarrhoeic lambs are the the reservoirs of STEC in this geographic region.
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