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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 3070-3077
DOI: https://doi.org/10.20546/ijcmas.2020.909.379


Epidemiology and Molecular Characterization of Shiga Toxigenic Escherichia coli from Diarrhoeic Lambs in Andhra Pradesh
Sujatha1, M. Srivani2*, K. V. Subramanyam3 and T. Srinivasa Rao4
1Veterinary Microbiology, Super Specialty Veterinary Hospital SVVU, Vishakapatnam, India
2Department of Veterinary Microbiology, 4Department of Veterinary Public Health, NTR College of Veterinary Science, Gannavaram, Krishna District, Andhra Pradesh, India
3Department of Veterinary Microbiology, College of Veterinary Science, Proddatur, Andhra Pradesh, India
*Corresponding author
Abstract:

A study was carried to investigate the epidemiology and molecular characterization of Shiga toxigenic Escherichia coli (STEC) from diarrhoeic lambs in Andhra Pradesh. A total of 212 faecal samples from diarrhoeic lambs of 1-7, 8-30, 31-60 and 61-90 days age groups were collected from Vizianagaram, West Godavari, Krishna, districts of Andhra Pradesh. The E. coli isolates were confirmed by cultural, biochemical and molecular methods. The virulence genes of STEC were detected using multiplex PCR. The results of the study revealed that the prevalence rate of E. coli in diarrhoeic lambs was 80.18% of which 87.05 % were identified as STEC.  Among the virulence genes of STEC studied, eaeA&hlyA genes were more prevalent (35.13%), followed by stx1 (12.83%) and stx2 (10.13%) genes. The combinations of stx1&eaeA, stx2& eaeA genes carrying isolates detected were 5.4% and 8.1%, respectively. Only four (2.70%) STEC isolates carried all the four (stx1, stx2, eaeA and hlyA) genes. This study indicated that diarrhoeic lambs are the the reservoirs of STEC in this geographic region. 


Keywords: Epidemiology, Shiga toxigenic coli, Diarrhoeic lambs
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How to cite this article:

Sujatha, T., M. Srivani, K. V. Subramanyam and Srinivasa Rao, T. 2020. Epidemiology and Molecular Characterization of Shiga Toxigenic Escherichia coli from Diarrhoeic Lambs in Andhra Pradesh.Int.J.Curr.Microbiol.App.Sci. 9(9): 3070-3077. doi: https://doi.org/10.20546/ijcmas.2020.909.379