National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
To study the approach of immunohistochemistry (IHC) in comparison with study of pathomorphological alterations, for diagnosis of rabies, 50 brain samples from different animals like dog, cattle, buffalo and mongoose suspected for rabies were collected at post mortem hall. Out of the 50 animal brain samples tested, 30 (60%) were confirmed to be rabid by Fluorescent Antibody Technique (FAT). The 30 positive brain samples were subjected for comparison of histopathology and immunohistochemistry. Sensitivity of diagnosis of rabies by histopathology and IHC was 88.23% and 93.75% respectively. The specificity of diagnosis of rabies by histopathology and IHC was 100%each whereas the accuracy of diagnosis of rabies by histopathology and IHC was 92.59%and 96.15% respectively. The immunohistochemistry is more sensitive than histopathology for diagnosis of rabies in formalin fixed brain samples. It is, therefore, recommended to supplement histopathology with IHC as an alternative diagnostic technique especially in the laboratories that resort to histopathology for diagnosis of rabies, in absence of facilities for immunofluorescence.
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