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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 2088-2095
DOI: https://doi.org/10.20546/ijcmas.2020.909.260


Identification and Characterization of Escherichia coli Isolates in Bovine Mastitis Milk from Bhubaneswar, Odisha, India
Kailash Singh Bisht1*, Rajashree Mishra1, Prasad Kumar Pati2,Ritun Patra3, Prasana Kumar Rath4 and Pravas Ranjan Sahoo5
1Department of Veterinary Microbiology, 2Department of Livestock Products Technology, 3Department of Veterinary Anatomy& Histology, 4Department of Veterinary Pathology, 5Department of Veterinary BiochemistryCollege of Veterinary Science and Animal Husbandry, OUAT, Bhubaneswar, Odisha-751003, India
*Corresponding author
Abstract:

Escherichia coli is one of the most commonly infecting bacterial etiology of the Bovine mastitis. The coliform mastitis is the most difficult type to treat due to increase in antibiotic resistance and generally cause recurrent and chronic form of mastitis. Therefore this present study was done to determine the prevalence rate of E. coli from the bovine mastitis milk and its antibiogram status. 250 mastitis milk samples were collected from the bovines. 27.2% (n= 68) E. coli isolates were identified in the samples. The isolated E. coli also showed biofilm forming ability in 51 isolates using Congo red agar method. Gentamicin is most sensitive drugs against E. coli isolates whereas ampicillin and tetracycline showed highest resistance. For the biofilm forming isolates, gentamicin is most effective whereas ampicillin followed by tetracycline and ofloxacin showed higher ineffectiveness. It was evident that the sessile form of E. coli isolates are more resistant to antibiotics compared to their planktonic forms. The multidrug resistance in E. coli isolates was also reported.


Keywords: Escherichia coli, Mastitis, Antibiogram status Ofloxacin
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How to cite this article:

Kailash Singh Bisht, Rajashree Mishra, Prasad Kumar Pati, Ritun Patra, Prasana Kumar Rath and Pravas Ranjan Sahoo. 2020. Identification and Characterization of Escherichia coli Isolates in Bovine Mastitis Milk from Bhubaneswar, Odisha.Int.J.Curr.Microbiol.App.Sci. 9(9): 2088-2095. doi: https://doi.org/10.20546/ijcmas.2020.909.260