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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 1867-1873
DOI: https://doi.org/10.20546/ijcmas.2020.909.235


Single Dose and Multi Dose Screening Assay of Entomopathogenic Fungi against Rice Leaf Roller, Cnaphalocrocis medinalis Guen
Malini Nilamudeen* and K. Sudharma
Department.of Agriculture. Entomology, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala, India
*Corresponding author
Abstract:

Entomopathogenic fungi are recognized as important biocontrol agents of insect pests of crops.  For a quantum leap in their adoption for pest management, virulent isolates against target pests are requisites.  It is also necessary to garner information on their pathogenicity and effective field dose. Single dose assay of all the nine fungal isolates at 108 spores ml-1 against third instar larvae of C. medinalis, showed that B. bassiana (Bb 5) which recorded cent per cent mortality at eight days after treatment was significantly superior to other treatments, and it was followed by B. bassiana (Bb 21), M. anisopliae (Ma 4) and A. flavus (Af-m1) with 90.00, 73.33 and 66.67 per cent mortality, respectively. The lethal concentration of B. bassiana (Bb 5), required to bring fifty per cent mortality at four days after treatment was 1.70 x 108spores ml-1against C. medinalis.  The corresponding LC90 values was 3.50 x 108spores ml-1.The LT50 recorded was 5.38 days for B. bassiana (Bb 5), against C. medinalis at a concentration of 108spores ml-1. The entomopathogenic fungus, B. bassiana (Bb 5) is found ideal for rice pest management.


Keywords: Entomopathogenic fungi, Single dose screening assay, Multi dose screening assay
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How to cite this article:

Malini Nilamudeen and Sudharma, K. 2020. Single Dose and Multi Dose Screening Assay of Entomopathogenic Fungi against Rice Leaf Roller, Cnaphalocrocis medinalis Guen.Int.J.Curr.Microbiol.App.Sci. 9(9): 1867-1873. doi: https://doi.org/10.20546/ijcmas.2020.909.235