Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 1514-1521
DOI: https://doi.org/10.20546/ijcmas.2020.909.192


Effect of Feeding Azolla on Sexual Behaviour, Seminal Characteristics and Freezability in Marwari Stallions
Manish Songara1, Arun Kumar Jhirwal2, R. A. Legha4, Yash Pal3, S. C. Goswami5,K. Ravi6, T. R. Talluri7*, Praveen Kumar Kaushik8 and Geetesh Mishra9
1VUTRC, Lunkaransar, RAJUVAS, India
2Dept. Livestock Production and Management, RAJUVAS, Bikaner, India
3ICAR-NRCE, India
4ICAR-NRC on Equines, Bikaner, Rajasthan, India
5Department. Livestock Production and Management, 9CVAS, RAJUVAS, Bikaner, India
6ICAR-Central Island Agricultural Research Institute (CIARI),
Andaman & Nicobar Islands, India
7Equine Production Campus, ICAR-NRC on Equines, Bikaner, Rajasthan, India
8Directorate of Gopalan, Rajasthan, Jaipur, India
*Corresponding author
Abstract:

The present investigation was carried out to evaluate the effect of feeding of Azollaon sexual behaviour, seminal characteristics and freezability in Marwari Stallions. Three adult fertile Marwari stallions were subjected to a switchover technique of feeding in which they were fed with basal feed for 45 days (T0), then for next 45 days feeding trial 10% of total concentrate feed protein was replaced by Azolla supplementation (T1). All the parameters were recorded during control and treatment feeding trials. Sexual behaviour and libido (erection time, ejaculation time, mounting time and number of thrust) were recorded at the time of semen collection and after exposing to the estrus mare. Six ejaculates from each stallion (18 in total) were collected during the breeding season for control and treatment stallions using AV and estrus mare as dummy. Immediately after collection, semen samples were evaluated for various quantitative and qualitative parameters. Then semen samples were diluted with primary (Citrate) extender 5% (v/v) and extended in secondary (Lactose-Glu) extender having Dimethyl formamide as cryoprotective agent and 20% (v/v) egg yolk. After equilibration, semen samples were filled in 0.5ml straws and frozen for 12 min and 4 cm above the liquid nitrogen and finally shifted into liquid nitrogen container. Post thaw seminal characteristics were studied to find out the effect of Azolla feeding on the freezability. From the above study it was observed that, there was no significant increase either in the libido or seminal characteristics of the stallions in either of the groups, but it was proved that Azolla can be used as an alternative source of protein in the diet of stallions in conserving the freezability and retaining the qualitative seminal parameters in stallions without deterioration.


Keywords: Azolla, Stallion semen, Freezability, Libido, Semen cryopreservation
Download this article as Download

How to cite this article:

Manish Songara, Arun Kumar Jhirwal, Legha RA, Yash Pal, SC. Goswami, SK. Ravi, TR. Talluri, Praveen Kumar Kaushik and Geetesh Mishra. 2020. Effect of Feeding Azolla on Sexual Behaviour, Seminal Characteristics and Freezability in Marwari Stallions.Int.J.Curr.Microbiol.App.Sci. 9(9): 1514-1521. doi: https://doi.org/10.20546/ijcmas.2020.909.192