National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was carried out to evaluate the effect of feeding of Azollaon sexual behaviour, seminal characteristics and freezability in Marwari Stallions. Three adult fertile Marwari stallions were subjected to a switchover technique of feeding in which they were fed with basal feed for 45 days (T0), then for next 45 days feeding trial 10% of total concentrate feed protein was replaced by Azolla supplementation (T1). All the parameters were recorded during control and treatment feeding trials. Sexual behaviour and libido (erection time, ejaculation time, mounting time and number of thrust) were recorded at the time of semen collection and after exposing to the estrus mare. Six ejaculates from each stallion (18 in total) were collected during the breeding season for control and treatment stallions using AV and estrus mare as dummy. Immediately after collection, semen samples were evaluated for various quantitative and qualitative parameters. Then semen samples were diluted with primary (Citrate) extender 5% (v/v) and extended in secondary (Lactose-Glu) extender having Dimethyl formamide as cryoprotective agent and 20% (v/v) egg yolk. After equilibration, semen samples were filled in 0.5ml straws and frozen for 12 min and 4 cm above the liquid nitrogen and finally shifted into liquid nitrogen container. Post thaw seminal characteristics were studied to find out the effect of Azolla feeding on the freezability. From the above study it was observed that, there was no significant increase either in the libido or seminal characteristics of the stallions in either of the groups, but it was proved that Azolla can be used as an alternative source of protein in the diet of stallions in conserving the freezability and retaining the qualitative seminal parameters in stallions without deterioration.
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