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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 1448-1454
DOI: https://doi.org/10.20546/ijcmas.2020.909.184


Studies on Effect of Temperature and pH on  Fusarium oxysporum f.sp. vasinfectum causing Okra Wilt
Hanumanthe Gowda*, P. R. Ramesh and J. M. Prashanth
1Krishi Vigyan Kendra (IIHR), Hirehalli, Tumkuru Distrct-571168, India
2Department of Plant Pathology, UAS, GKVK, Bengaluru-65, India
*Corresponding author
Abstract:

The present study was undertaken to find out the different temperature levels and pH range suitable for growth of twenty five isolates of Fusarium oxysporum f. sp. vasinfectum collected and isolated from different locations. The temperature of 30°C and 25°C was found best for the growth of all the isolates. At these temperatures, there was significant difference between the isolates in their growth, whereas temperature below 25°C and above 30°C reduced the growth of isolate drastically and least growth was recorded both in solid and liquid media at 15°C. Isolates like MYS-15 and MYS-16 showed mean maximum growth with colony diameter of 58.90 mm and 54.81mm, respectively and found significantly superior over other isolates. Based on the growth on liquid medium with different temperature levels, isolates were categorized in to five groups. Among them none of the isolate fell under group I and group V. Eleven isolates fell under group II. Twelve isolates fell under group III (medium growers) Whereas, remaining two isolates fell under group IV (fast growers) with mean dry mycelial weight ranged from 450.1 mg to 600 mg. Hydrogen ion concentration (pH) 7.0 and 6.0 were found better for all the F. oxysporum f. sp. vasinfectum isolates with mean maximum growth of 64.37 mm and 57.43 mm colony diameter on solid media and 552.86 and 503.24 mg dry mycelial weight on liquid media respectively.


Keywords: Fusarium oxysporum f. sp. vasinfectum, temperature, pH Culture media, Okra, Colony growth
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How to cite this article:

Hanumanthe Gowda, B., P. R. Ramesh and Prashanth, J. M. 2020. Studies on Effect of Temperature and ph on Fusarium oxysporum f.sp. vasinfectum causing Okra Wilt.Int.J.Curr.Microbiol.App.Sci. 9(9): 1448-1454. doi: https://doi.org/10.20546/ijcmas.2020.909.184