International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 396-404

Effect of Tissue Culture in Vegetable Improvement
Amba Kumari1*, Dibyanshu Shekhar1, M. S. Kundu2,Ram Prawesh Prasad1, A. P. Rakesh1 and Chandan Kumar1
1Krishi Vigyan Kendra, Jale, Darbhanga, Bihar-847302, India
2Dr.Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar-848125, India
*Corresponding author

As an emerging technology, the plant tissue culture has a great impact on agriculture, through providing plants needed to meet the ever increasing world demand. It has made significant advancement in the field of vegetable crops. Plant tissue culture represents the most promising areas of application at present time and giving an outlook into the future. All biotechnological approaches like genetic Meristem culture, haploid induction or Somatic hybridization in the field of vegetable crops to improve traits strongly depends on an efficient in-vitro plant regeneration system. Potato viruses are major causes of yield loss and reduction in quality of seed tubers. Meristem culture with thermotherapy applied all three viruses PVX, PVS, PLRV eliminated with success rate of 86%, 100% and 83% respectively (Biniam and Tadesse, 2007). Many sources of useful genes cannot be included in crop improvement because of sexual incompatibilities, while it can be readily achieved by somatic hybridization in potato (Tiwari et al., 2011). Tomato is highly prone to biotic stress, especially diseases, insects and nematodes. Tissue culture technology can be used with the aim of transfer of important traits from the wild species to the already cultivated and popular species through somatic hybridization (Kharkongar et al., 2013).Cryopreservation is done for long term preservation of important planting material to minimize time and cost on manpower, chemicals. Cryopreservation has done in different vegetables e.g. shoot primordial of melon by slow freezing (Ishikawa et al., 1996). Apical meristem of garlic by vitrification (Niwata et al., 1995). The tissue culture techniques enable to produce homozygous plants in relatively short  time period through the protoplast fusion, anther and microspore culture instead of conventional breeding( Hussain et al., 2012). Current and future status of plant tissue culture in vegetable crops are production of improved varieties, conservation of endangered species, production of secondary metabolites or plant-made vaccines or antibodies through cell suspension culture on large scale. Providing planting material of plants whose multiplication is not possible through seed to meet the ever increasing world demand.

Keywords: In-vitro plant regeneration, Vegetable crops, Somatic hybridization
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Amba Kumari, Dibyanshu Shekhar, M. S. Kundu, Ram Prawesh Prasad, A. P. Rakesh and Chandan Kumar. 2020. Effect of Tissue Culture in Vegetable Improvement.Int.J.Curr.Microbiol.App.Sci. 9(9): 396-404. doi: