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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 345-350
DOI: https://doi.org/10.20546/ijcmas.2020.909.044


Diagnosis of Babesiosis by Conventional Microscopy and PCR
Sai Sindhu1*, B. Shobhamani1, K. Suresh2 and V. Chengalva rayulu3
1Department of Veterinary Medicine, 3Department of Veterinary Parasitology,
College of Veterinary Science, Tirupati, India
2Department of Veterinary Clinical Complex, N.T.R College of Veterinary Science, Gannavaram, India
*Corresponding author
Abstract:

The present study was conducted in Veterinary Clinical Complex, College of Veterinary Science, Tirupati over a period of nine months to diagnose the Babesia infection. 235 dogs suspected for babesiosis were collected and subjected for diagnosis using conventional and molecular techniques. Out of 235 samples examined by conventional microscopy of Giemsa stained blood smears revealed eleven positive samples which includes Babesia gibsoni (10) and Babesia canis (1) whereas with polymerase chain reaction 20 samples showed amplicons of 671 bp and 3 samples showed amplicons of 450 bp indicative of Babesia gibsoni and Babesia canis respectively.


Keywords: Babesia gibsoni, Babesia canis, Diagnosis, Microscopy, Polymerase chain reaction
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How to cite this article:

Sai Sindhu, B., B. Shobhamani, K. Suresh and Chengalva Rayulu, V. 2020. Diagnosis of Babesiosis by Conventional Microscopy and PCR.Int.J.Curr.Microbiol.App.Sci. 9(9): 345-350. doi: https://doi.org/10.20546/ijcmas.2020.909.044