Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
Home Editorial Board Current Issue Special Issue Archives Indexing Submit Article
Instruction to Authors Manuscript Charges Copyright Form Model Manuscript Process Flow (Time Frame) FAQ
Reviewer Guidelines Selection of Reviewers Join our Team of Reviewers Apply as Reviewer Reviewer Login
Editorial Review Policy Embargo Policy Ethical Expectations Consent For Identifying Information Informed Consent Manuscript Withdrawal Policy Peer Review Process Plagiarism Policy Ethical Violations Appeal Policy Against Editorial Decision Advertising Policies Authorship Policy
Access Policies Authorship Publication Policy Publication Ethics Complaints Policy Confidential Information Conflicts of Interest Policy Consent for Copyrighted Material Copyright Policy Corrections and Retractions Digital Preservation Policy Research On Human And Animal Studies Open Access Policy
Publication Ethics Contact
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Join as a Reviewer

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
Announcement

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 9, Issue:9, September, 2020
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
Metrics and citations

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 345-350
DOI: https://doi.org/10.20546/ijcmas.2020.909.044


Diagnosis of Babesiosis by Conventional Microscopy and PCR
Sai Sindhu1*, B. Shobhamani1, K. Suresh2 and V. Chengalva rayulu3
1Department of Veterinary Medicine, 3Department of Veterinary Parasitology,
College of Veterinary Science, Tirupati, India
2Department of Veterinary Clinical Complex, N.T.R College of Veterinary Science, Gannavaram, India
*Corresponding author
Abstract:

The present study was conducted in Veterinary Clinical Complex, College of Veterinary Science, Tirupati over a period of nine months to diagnose the Babesia infection. 235 dogs suspected for babesiosis were collected and subjected for diagnosis using conventional and molecular techniques. Out of 235 samples examined by conventional microscopy of Giemsa stained blood smears revealed eleven positive samples which includes Babesia gibsoni (10) and Babesia canis (1) whereas with polymerase chain reaction 20 samples showed amplicons of 671 bp and 3 samples showed amplicons of 450 bp indicative of Babesia gibsoni and Babesia canis respectively.


Keywords: Babesia gibsoni, Babesia canis, Diagnosis, Microscopy, Polymerase chain reaction

Download this article as Download

How to cite this article:

Sai Sindhu, B., B. Shobhamani, K. Suresh and Chengalva Rayulu, V. 2020. Diagnosis of Babesiosis by Conventional Microscopy and PCR.Int.J.Curr.Microbiol.App.Sci. 9(9): 345-350. doi: https://doi.org/10.20546/ijcmas.2020.909.044
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

Citations
Home | Terms of Use | Copyright Form 2012-2020 Copy rights reserved IJCMAS. Powered by ThinkNEXT Technologies