National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pigeonpea is the major source of dietary protein for vegetarian population in India and other developing countries. The following study was taken to understand the genetic relationship between genotypes on the basis of protein profiling of leaves and seed protein. Ten genotypes of Pigeon pea [Cajanus cajan (L.) Millspaugh] including one wild species was sown in the field at Student Instructional Farm, ANDUA & T Kumarganj Ayodhya. Total protein was extracted using 0.1 M Sodium phosphate buffer (pH 7.2) containing NaH2PO4 and Na2HPO4. Protein was electrophoresed on 12% SDS- PAGE along with standard protein marker and detected by commassive brilliant blue staining. Pigeonpea leaves and seeds protein showed variability in banding pattern of polypeptide on gel. RM values of different pigeonpea genotype were measured. The results showed that both leaves and seeds protein migrated between a range of < 10 to 250 kDa. NDA-3, MAL-6 and MAL-13 had minimum protein bands (8 bands) while maximum protein bands was observed in NDA-14-6 (12 bands) in leaves. In protein profile of seeds it has been seen that Cajanus scarabaeoides showed maximum bands (20 bands) while NDA-2 showed minimum protein band (11). All the accessions commonly showed at least six major bands in leaves while 11 major bands in seeds. In this context we can say that seeds of wild genotype contain more protein than cultivated pigeonpea genotype. The main objective of this study is to reveal intra-specific similarities and genetic diversity in protein content amongst ten genotypes of pigeon pea.
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