National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Earliness, an adaptive trait and factor of variation for agronomic characters, is a major trait in plant breeding. In present investigation, the experimental material comprised of P1, P2, F1, F2 and F2:3 generations of wheat crossDL-788-2 X GW-322 for earliness related traits with objective of linkage and QTL mapping in bread wheat. Out of 200 SSR markers screened for parental polymorphism for earliness related traits, only 11% of SSR markers showed good polymorphism between two parental lines. Out of 22 tests, all the test markers showed non-significant chi-square which revealed that observed data were agreement with expected ratio of 1:2:1 segregation ratio. The linkage map was constructed using software Ici Mapping v.4.1 and recombination frequencies were converted into map distance using Kosambi’s mapping function. The markers were grouped with minimum logarithm of the odds (LOD) of 3.0 with walking speed was set at 1.0 cM. Four linkage groups with a total map length of 267.12 cM were constructed using data from 22 marker loci for 74 F2 plants that ranged from minimum of 8.62 cM (LG4) to maximum of 126.56 cM (LG1).Genotypic data of F2 and phenotypic data of on 74 F2:3 lines were analyzed for identification of the main effect QTLs using the software ICIM-ADD mapping in QTL IciMappingV4.1. A linkage map of earliness related traits output data file was used for the construction of QTL mapping. One QTL was identified for days to 50% flowering (LG1 at 58.0 cM, LOD 3.06, 18 PVE %) and two QTLs for days to maturity (LG1 at 21 cM, LOD 8.89, 31.51 PVE% and LG3 at 38 cM, LOD 12.83, 45.16 PVE%).with use of molecular marker and QTL mapping complex from of earliness traits and their underlying genes are now far more accessible which can be routinely used by breeders in marker assisted selection in wheat breeding programs.
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