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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:8, August, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(8): 3404-3414
DOI: https://doi.org/10.20546/ijcmas.2020.908.393


Effects of Freeze Drying on Antioxidants and Immunoglobulins Level of Zebu Bovine Colostrum
Dushyant Kumar Sharma*, Debabrata Mondal, R. Raguvaran, Arvind Kumar Das and Narayani Yadav
Division of Medicine, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, U.P. India
*Corresponding author
Abstract:

The aim of present study was to investigate the effect of freeze drying on total antioxidant and immunoglobulin levels of bovine colostrum in desi breed (Zebu cattle). A total of 12 colostrum samples were collected from the desi breed (Zebu cattle) reared at LPM section of IVRI, Izatnagar. Collected colostrum was converted aseptically into dry powder form by freeze drying at (-) 40º C with low pressure. Antioxidant content was analysed using free radical scavenging activity (DPPH assay) and total antioxidant capacity (FRAP assay).Qualitatively and quantitative immunoglobulin level was assessed by zinc sulphate turbidity and IgG estimation respectively. Average reduction in DPPH scavenging activity was found to be 35.04 % on freeze drying whereas average reduction of FRAP value was found to be 14.96 % on freeze drying. Immunoglobulin content of bovine colostrum was decreased by 23.86 % of ZST unit after freeze drying whereas with respect to IgG level, the average percentage reduction was 26.05 % after freeze drying of bovine colostrum. Present study concludes that freeze drying reduces antioxidants and immunoglobulin level of bovine colostrum.


Keywords: Freeze drying, Bovine colostrum, Antioxidant, Immunoglobulins, Zebu

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How to cite this article:

Dushyant Kumar Sharma, Debabrata Mondal, R. Raguvaran, Arvind Kumar Das and Narayani Yadav. 2020. Effects of Freeze Drying on Antioxidants and Immunoglobulins Level of Zebu Bovine Colostrum.Int.J.Curr.Microbiol.App.Sci. 9(8): 3404-3414. doi: https://doi.org/10.20546/ijcmas.2020.908.393
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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