National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An experiment was carried out at AICRP on Potato under Odisha University of Agriculture and Technology, Bhubaneswar to study diversity among morphologically distinguishable potato genotypes with SSR markers. Twenty potato genotypes were laid out in a Randomized Block Design with four replications. Eight morphological characters were observed based on DUS characteristics to visually differentiate genotypes. In addition, fifteen SSR markers were used to depict clear-cut variation among genotypes with the aid of polymorphic bands. A total of 51 loci were amplified by 15 SSR markers which exhibited 92.16 percent polymorphism. The PIC values varied widely among 15 SSR loci tested and ranged from a minimum 0.2078 (STM1106) to maximum 0.7756 (STM0019) with an average of 0.5200. Heterozygosity values ranged from minimum 0.2659 (STM1106) to maximum 0.8047 (STM0019). The Jaccard’s dissimilarity coefficient was found to vary from 0.321 to 0.628. The binary data were used to calculate genetic dissimilarities based on Jaccard’s coefficient and UPGMA (Unweighted Pair Group Method using Arithmetical Means). A dendrogram was constructed using DARwin version 6 software, exhibiting pictorial expression of diverse genotypes. UPGMA divided the populations of 20 genotypes into six clusters; Cluster IV being the largest containing seven genotypes. Kufri Lima was found to be the most divergent from rest of the genotypes.
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