National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Cowpea (Vigna unguiculata) is a legume crop growing in almost all parts of world including tropics and subtropics. This study was thus undertaken to assess genetic diversity among the different parents used for hybridization and confirmation of hybrids of cowpea (Vigna unguiculata) by using randomly amplified polymorphic DNA (RAPD) markers. RAPD profiles for 11 genotypes were generated with 20 random decamer primers. Out of 20 primers screened 15 primers gave scorable DNA fragments and each of the 15 primers revealed various levels of polymorphism. These primers generated 476 DNA fragments in the average range of 167bp to 3300bp, of which 367 were polymorphic. The percentage of polymorphism ranged from 35.29 to 100% with an average of 79.96%. The overall range of similarity among 11 genotypes was found to be very wide, ranging from 0.328 to 0.613 which indicates there was high variability among the cowpea genotypes under study. The RAPD primer OPA-18 was effectively used for confirmation of hybridity.
Citations |
 |
 |
 |
 |
