National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Silkworm gut bacterial isolates were identified by using 16S rRNA gene sequence data analysis. Total viable count, spore count and spore percentage were tested on selective NA medium and recorded at different hours interval. The highest value of total viable count was recorded (420 x 108CFU/ml), spore count was higher (100 x 108CFU/ml) after 72 h of inoculation. The sporulation percentage of B. Subtilis (7.69, 10.20, 23.8 and 37.30) was found to gradually increase from 24 h to 96 h respectively. Enhancement of sporulation process using in mixture, highest total viable counts (190 ×108CFU/ml) and highest heat resistant spore count (183 ×108CFU/ ml).The spore percentage was 96.3 per cent in mixture and lowest spore percentage was observed in CaCl2 (32 ×108CFU/ml). Bacteria survived even up to 180 days of storage with different survival percentage over the period. The highest survivable percentage (88.3%) of viable cell count was recorded on 30th day. Evaluated bioformulation against pest and disease in mulberry. In thrips, highest percentage of reduction over control was observed in 100per cent (86.95%) followed by 80 per cent (80.43%), 60 per cent (75.00%) and 40 per cent (64.13%). The endogenous bacterial isolates of B. subtilis and B. tequilensis were tested individually to assess the inhibition against the radial mycelial growth of M. phaseolina and F. oxysporum. The bacterial isolates of B. subtilis recorded the least mycelial growth (45.00 mm) against M. phaseolina. The least mycelial growth of F. oxysporum was recorded in treatment with B. subtilis (63.00mm).
Silkworm, Gut bacteria, Thrips, Macrophomina phaseolina, Fusarium oxysporum.
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