National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Broccoli (Brassica oleracea var. italica) is an important part of diet in India. Turnip mosaic virus (TuMV), member of the genus Potyvirus, is the most important virus of commercially grown broccoli in many Asian countries. Symptoms based survey of different backyard vegetable gardens during rabi season of 2015-16, revealed that the mean disease incidence of TuMV was 36.21 per cent. The occurrence of TuMV in Broccoli was confirmed by symptomatology, transmission electron microscopy (flexuous filamentous particles of 800 × 12 nm), DAS-ELISA, RT-PCR and partial characterization of cytoplasmic inclusion (CI) protein and coat protein (CP) domains. Phylogenetic analysis of the partial CP sequences of the new TuMV isolate (AR-Brc; KP876504) revealed their closest relationship with the members of the World-B genogroup of TuMV. Significant physiological changes were observed in diseased leaves in terms of chlorophyll, total sugar, reducing sugar, total phenol and total proteins. There was significant decrease in chlorophyll contents; chlorophyll-a (48.75%), chlorophyll-b (42.86%) and total chlorophyll (47.77%). Total protein content significantly increased in case of severe symptoms (22.55%) followed by mild symptoms (19.61%). There was a significant increase in the total sugar content in mild symptom (60%) followed by severe symptoms (20%). Significant increase in the reducing sugar content was also observed in mild symptoms (5.88%) whereas it decreased in severe symptoms (11.76%). Similarly, total phenol content also significantly increased in mild type (9.09%) whereas it decreased in severe symptoms (13.63%).
Citations |
 |
 |
 |
 |
