National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Two species of Listeria are pathogenic; L. monocytogenes infects humans and animals, and L. ivanovii infects ruminants only have been considered. While infection with L. monocytogenes and L. ivanovii are known as important causes of abortion and reproductive failure in ruminants and the role of nitric oxide (NO) expression in different organs of affected animals in India is limited. Therefore, the current study, (n= 78) serum and organs (comprising of 26 ovaries, 26 uterus and 26 brain) samples from the local abattoir in and around Jammu region were taken and evaluate for the presences of Listeria spp. by employing two fold enrichment procedure for isolation and confirmed by biochemical tests. Then further, pathogenicity of isolated were confirmed by Phosphatidylinositol-specific phospholipase C (PI-PLC) assay. Out of them L. monocytogenes was detected in 5 samples i.e. 3 in uterus, 1 in ovary and 1 in brain, and L. ivanovii was isolated only in 3 uterus sample with over-all prevalence of 6.41% and 3.85% respectively. The prevalence of other Listeria spp. in the sample was 3.85%. Further, we were found high concentration of NO in Listeria spp. infected samples in comparison to the Listeria spp. was not isolated. This study demonstrated that Listeria spp. (mainly L. monocytogenes and L. ivanovii) infected sheep showed very high NO concentration level compared to without infected animals. The functional role of nitric oxide (either iNOS or nNOS) needs to be further studied in natural and experimental models with Listeria spp. infection.
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