National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Brassica juncea is one of the most important oilseed crops of India, but its genetic diversity is still not explored properly. A better understanding on this topic is a prerequisite for the better utilization of genotypes for breeding programs as well as in crop improvement. This study was undertaken to identify the polymorphism among thirty six genotypes including pure lines/varieties/ accession lines of different agro-climatic areas using fifteen SSR markers. All the thirty six genotypes were raised in pots for extraction of genomic DNA from seven days old seedlings. Amplification of the genomic DNA was carried out using a fifteen primer pairs. Out of the fifteen primers tested, seven reported polymorphism and a total of 32 alleles were amplified. The number of alleles per primer varied from one to three, with an average of 1.5 fragments, while the size of the fragments ranged from 200bp to 400bp. Jaccard’s similarity coefficients based on SSR data ranged from 0.36 to 0. 1. The study focuses on using SSR markers as a stronger and reliable tool for diversity studies.
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