National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The enormosity of infectious diseases continues to be a major constraint to the economical viability of livestock industry. Combined vaccines against more than one infectious disease reduce the cost, time, and labour of vaccination. In present study, formalized vaccines against Haemorrhagic Septicaemia and Black Quarter diseases were prepared using P52 strain of Pasteurella multocida and Clostridium chauvoei strain 49. Different proportions of these two vaccines were mixed to formulate various groups of combined vaccines, which were subsequently standardized for sterility, safety and potency. Amongst all combinations a group containing 1 ml of H.S. casein sucrose yeast extract agar washed vaccine and 4 ml of B.Q. thioglycolate broth and culture vaccine with shaking was found most efficacious in our study. This combined vaccine conferred 100% protection against H.S. disease and 75% against B.Q. disease in rabbits and guinea pigs, respectively in potency test performed for individual vaccines. The study also revealed that casein sucrose yeast agar and fluid thioglycolate broth are better medium for cultivation of P. multocida and Cl. chauvoei. Enhanced growth of Cl. chauvoei was observed when incubated on a shaker as compared to static culture.
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