National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An experiment was conducted on culture establishment of Coccinia grandis (L.) through nodal segments under in vitro conditions with an objective to standardise a viable protocol for in vitro propagation. Significant results were observed for number of buds sprouted, percent of shoot bud induction, number of days taken for shoot initiation, number of shoots per explant, shoot length, number of leaves per shoot and vitrification For shoot induction and succeeding growth was effectively induced by TDZ at lower (0.5 mg l-1) concentration as compared to higher (1.0 mg l-1) concentration). For shoot proliferation 3.0 mg l-1 BAP + 0.25 mg l-1 NAA (T4) and 4.0 mg l-1 BAP + 0.25 mg l-1 NAA (T5) in MS medium were more efficient for inducing maximum number of micro shoots and leaves.
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