International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:7, July, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 2273-2286

Culture Establishment of Coccinia grandis (L.) through Nodal Segments under in vitro Conditions
Mohammad Amin1*, A. V. D. Dorajee Rao2, K. Ravindra Kumar3 and D. R. Salomi Suneetha4
1Department of Vegetable Science
2 ,3Department of Horticulture
4Department of Biochemistry, College of Horticulture, Dr. Y. S. R Horticultural University, Venkataramannagudem, West Godavari, India
*Corresponding author

An experiment was conducted on culture establishment of Coccinia grandis (L.) through nodal segments under in vitro conditions with an objective to standardise a viable protocol for in vitro propagation. Significant results were observed for number of buds sprouted, percent of shoot bud induction, number of days taken for shoot initiation, number of shoots per explant, shoot length, number of leaves per shoot and vitrification For shoot induction and succeeding growth was effectively induced by TDZ at lower (0.5 mg l-1) concentration as compared to higher (1.0 mg l-1) concentration). For shoot proliferation 3.0 mg l-1 BAP + 0.25 mg l-1 NAA (T4) and 4.0 mg l-1 BAP + 0.25 mg l-1 NAA (T5) in MS medium were more efficient for inducing maximum number of micro shoots and leaves.

Keywords: Coccinia, Nodal segments, Culture establishment, Shoot proliferation, In vitro

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How to cite this article:

Mohammad Amin, A. V. D. Dorajee Rao, K. Ravindra Kumar and Salomi Suneetha, D. R. 2020. Culture Establishment of Coccinia grandis (L.) Through Nodal Segments under in vitro Conditions.Int.J.Curr.Microbiol.App.Sci. 9(7): 2273-2286. doi:
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