National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Listeria monocytogenes is one of the major food contaminants with potential to cause lethal food poisoning in both humans and animals. Among different food borne pathogens, Listeria monocytogenes has a high mortality rate and is therefore considered one of the most dangerous foodborne pathogen. The present study was aimed at finding the prevalence of Listeria monocytogenes in raw chicken meat purchased from different retail outlets and local butcher shops across the Ludhiana city. In the present study a total of 100 raw chicken meat samples were collected (80 fresh raw samples and 20 frozen chicken meat products). During this study, 100 chicken meat samples were inoculated in PALCAM selective agar for the selective isolation of L. monocytogenes and were later characterized by a combination of microscopic and biochemicaltests. Results of the study revealed that02 samples were containing L. monocytogenes, which is 02% of the total samples. These positive samples were subjected to molecular characterization using standard PCR technique to attest their presence in these meat samples. The PCR technique was found to be more specific/sensitive, reliable, precise and rapid technique to supplement the conventional methods of diagnosis of the L. monocytogenes.
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