Entomopathogenic fungi are important natural enemies of arthropods and can be used for biological control of ticks. They are widely distributed in a wide range of habitats including aquatic, forest, agricultural soil and pasture habitats. Sixty crop roots soil samples were collected from nearby field and grazing areas of animals in Durg district of Chhattisgarh. A total of seven fungal isolates were recovered from the organic environment of Durg, Chhattisgarh and they were belonging to Genus; Aspergillus, Penicilium, Cladosporium, Fusarium, Rhizopus, Metarhizium and Trichoderma. The fungal colonies were isolated from soil samples and surface sterilized ticks were treated with aqueous fungal suspension. The mortality of ticks was observed on third to seventh day when hyphae covered the whole body surface. Out of the seven isolates explored from soil samples, four isolates were found to infect ticks namely, Fusarium sp., Rhizopus sp., Metarhizium sp. and Trichoderm sp.

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