National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
1Department of Agricultural Entomology, Tamil Nadu Agricultural University,Coimbatore-3, India
3Department of Sericulture, Forest College and Research Institute, Mettupalayam, India
Antibacterial activity of proteins eluted from immunized haemolymph collected from breeds of mulberry silkworm, Bombyx mori L., viz., Rong daizo, CSR2 and double hybrid were studied against bacterial pathogens. The breeds were immunized with bacterial pathogens, Escherichia coli (KR strain), Bacillus thuringiensis and Staphylococcus aureus . In vitro efficacy of purified antibacterial proteins from breeds of B.mori against E.coli, S. aureus and B. thuringiensis revealed that among the ten fractions of antibacterial proteins eluted from B.mori breeds, ninth fraction@ 200µl showed higher antibacterial activity in Rong daizo (3.01cm, 3.10 cm and 1.82cm) (2.71 cm, 3.01cm and 1.60 cm), CSR2 (2.98 cm, 3.05 cm and 1.81 cm) and double hybrid ( 2.89 cm, 2.98 cm and 1.79 cm ) against E.coli, S. aureus and B. thuringiensis (Table 1,2&3).Lesser inhibitory activity was recorded in seventh fraction @ 200 µl concentration of Rong daizo (2.71 cm, 3.01 and 1.60 cm ) followed by CSR2 (2.68 cm, 2.98cm and 1.59 cm) and DH (2.69 cm, 2.96 cm and 1.54 cm) respectively. Standard antibiotic streptomycin sulphate @ 200 µl concentration produced the inhibition zone of 7.12 cm, 8.12 cm and 6.32 cm. Purified antibacterial protein fractions showed higher antibacterial activity against gram negative E.coli and gram positive S.aureus and low activity against B.thuringiensis.
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