National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In the current study we analysed the effect of 0.1% mercuric chloride (HgCl2) on surface sterilization of the explants at different durations of time (1-5mins). It was observed that, longer the duration of treatment, lower was the bacterial contamination (8% contamination at 5 min duration of HgCl2 treatment) but the survival percentage of the culture decreased as exposure time increased (35% at 5 min). 3 min of exposure gave the best result for both bacterial contamination (18%) and survival percentage (68%) among other time durations. Fungal contamination doesn’t showed any significant difference at different time intervals.
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