National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Canine Mammary Tumour (CMT) is the most common malignancy of unspayed female dogs and is of potential importance as a model for human breast cancer. Mortality rate in female dogs is three times than that of human females with breast cancer due to lack of detection at an early stage. Therefore it is important to identify candidate biomarkers for canine mammary tumour with potential for disease diagnosis at early stages. Tumour antigen P53 (TP53) is well established marker for human breast cancer studies, however few studies have focussed on its characterization in CMTs. Therefore in this study immunodominant region in TP53 gene was amplified and cloned in prokaryotic expression vector. Further the conditions were optimized for the expression of the recombinant protein. The recombinant protein was purified using Ni-NTA affinity chromatography. The hyperimmune sera were raised in rabbits against the recombinant protein. In the hyperimmune sera, antibodies specific to TP53 were detected by Indirect ELISA, confirming the serological reactivity of the immunodominant region. The antibodies raised against canine TP53 will be useful in developing assays for detecting TP53 antigen in serum of dogs with cancers. Further the purified antibodies can also be utilized for detection of TP53 antigen in CMT tissues.
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