The flacherie diseased silkworms may contain multiple species of bacteria viz., Streptococcus faecalis, Staphylococcus aureus, Serratia marcescens and Bacillus sp. The use of botanical formulations possessing antibacterial activity are preferred in silkworm rearing over the chemical agents which found to be effective on checking bacterial infection and enhance the growth of silkworm. Different forms and methods of application of Adhatoda vasica was found effective in the management of bacterial flacherie of silkworm. In the present study, three bacterial species (Bacillus sp., Staphylococcus sp. and Streptococcus sp.) were isolated from haemolymph of silkworm and used under in-vitro condition. The study revealed that, the aqueous, acetone and alcoholic extract of A.vasica at different concentrations (2, 4 and 6 %) were highly effective against three bacterial species. However, the maximum zone of inhibition was observed in alcoholic extract (8.00, 9.12 and 8.99, 9.62 mm)(7.42, 7.88 and 7.84, 8.63 mm) (8.25, 8.87 and 8.84, 9.21 mm) and minimum in aqueous extract(7.66, 8.46 and 8.25, 8.94 mm) (6.96 and 7.29., 7.35 and 7.79 mm) (6.46, 6.92 and 8.09, 8.46 mm) on 24 and 48 hours of incubationagainst10-5 and 10-7 spore dilution of Bacillus, Staphylococcus and Streptococcus species, respectively.

Download this article as